Of the three factors (pH, temperature, or concentration of enzymes) affecting enzyme activity you examined in the simulations, pick one and write a brief lab report (following the same lab report format as before) summarizing the steps of the experiment and reporting your conclusions.Laboratory Simulation
2/6/21, 4:52 PM
Student: Jessie
My Hypothesis:
Enzyme activity rises up to a certain pH value and then decreases.
My Strategy:
I will prepare test tubes with the same amount of enzyme at different pH, and I will measure the height of the bubbles.
My Conclusion:
The reaction rate is fastest near pH 7 and slower at higher and lower pH.
Collected Lab Data
Phase
pH
Volume of Bubbles (mL)
2
7
2.5
3
5
1
4
3
0.5
5
9
2
6
11
0.7
Saved Graphs
Graph 1
3
Bubble Volume (mL)
2.5
2
1.5
1
0.5
0
3
5
7
9
11
pH
Graph 2
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/simulations/1.9.0/how-enzymes-function/ph/sim-lab/lab-phases.html
Page 1 of 2
Laboratory Simulation
2/6/21, 4:52 PM
3
Bubble Volume (mL)
2.5
2
1.5
1
0.5
0
3
5
7
9
11
pH
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/simulations/1.9.0/how-enzymes-function/ph/sim-lab/lab-phases.html
Page 2 of 2
Laboratory Simulation
2/6/21, 5:04 PM
Student: Jessie
My hypothesis:
Increasing temperature will increase the reaction rate, up to a certain temperature.
My strategy:
I will measure the height of the bubble column in tubes in which the same amount of enzyme is prepared at different temperatures.
My conclusion:
As temperature increases, the reaction rate increases to a point. After a maximum rate, the reaction slows down as temperature increases.
Collected Lab Data
Phase
Catalase Temperature (°C)
Volume of Bubbles (mL)
2
25
2
3
00
0.5
4
37
4
5
45
3
6
55
1.5
Saved Graphs
Graph 1
5
4.5
Bubble Volume (mL)
4
3.5
3
2.5
2
1.5
1
0.5
0
0
25
37
45
55
Temperature of Catalase (°C)
Graph 2
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/si…lations/1.9.1/how-enzymes-function/temperature/sim-lab/lab-phases.html
Page 1 of 2
Laboratory Simulation
2/6/21, 5:04 PM
5
4.5
Bubble Volume (mL)
4
3.5
3
2.5
2
1.5
1
0.5
0
0
25
37
45
55
Temperature of Catalase (°C)
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/si…lations/1.9.1/how-enzymes-function/temperature/sim-lab/lab-phases.html
Page 2 of 2
Laboratory Simulation
2/6/21, 4:28 PM
Student: Jessie
My Hypothesis:
Increasing enzyme concentration decreases the reaction rate.
My Strategy:
I will measure the height of bubbles in tubes with different enzyme concentrations.
My Conclusion:
Adding more catalase speeds up the reaction.
Collected Lab Data
Phase
Catalase Concentration (%)
Volume of Bubbles (mL)
2
5
2
3
10
4
4
5
6
0
5
0
Indicates incorrect data
Saved Graphs
Graph 1
8
Bubble Volume (mL)
7
6
5
4
3
2
1
0
0
5
5
10
Catalase Concentration (%)
Graph 2
8
Bubble Volume (mL)
7
6
5
4
3
2
1
0
0
5
5
10
Catalase Concentration (%)
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/si…tions/1.9.0/how-enzymes-function/concentration/sim-lab/lab-phases.html
Page 1 of 2
Laboratory Simulation
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/si…tions/1.9.0/how-enzymes-function/concentration/sim-lab/lab-phases.html
2/6/21, 4:28 PM
Page 2 of 2
Laboratory Simulation
2/6/21, 5:13 PM
Student: Jessie
Hypothesis:
Only speci,c combinations of an enzyme and substrate will exhibit a reaction.
Strategy:
I will examine different combinations of chemicals with and without enzymes.
Conclusion:
Catalase and hydrogen peroxide
Collected Lab Data
Catalase and H 2 O 2
Catalase and Water
H 2 O 2 and Water
Reactive
Not Reactive
Not Reactive
https://vlsimulation.learningtools.prod.mheducation.com/virtual-labs/simulations/1.9.0/how-enzymes-function/activity/sim-lab/lab-phases.html
Page 1 of 1
Suggested format for lab reports:
Organize your lab report logically, and give each section a title. Sections of a lab report typically
include an introduction, methods, results, and discussion. Also be sure to cite any
references used (you can use whatever citation format you like, just be sure it’s consistent).
There are not strict formatting requirements, however please write your intro and discussion
in paragraph form. The other sections can be written up however you like. Avoid weird fonts
and distracting colors. You can include other sections if you want, but I don’t recommend
omitting any of the four sections specified above. There is no length requirement ­ A page should
suffice for most lab reports. Feel free to be creative and play around with graphs!
Your report should include at minimum:
● a brief introduction (in paragraph form)
● a testable hypothesis that represents a plausible explanation for the observation(s)
● explanation of the steps of a repeatable experiment designed to test the hypothesis
● variables: independent, dependent, standardized variables and experimental control(s)
● summary of results (could just be a data table)
● a discussion (in paragraph form) in which you interpret your results ­ Hypothesis
supported? Or should it be rejected? Sources of error? Future studies?
If you need an example, your report could be structured like this (you can use this as a template):
Lab (#) ­ (Subject of Lab)
(Name)
(Date)
Introduction
(This is a short introduction to the topic of the lab. This is where you should include a little
background information, your hypotheses (null + alternative), and purpose for the experiment.
This section should be written in paragraph form, rather than as a bulleted outline or
something.)
Methods
(List the materials you used (or pretended to use in the LearnSmart simulation) and the steps
of the experiment. Talk about your variables and controls here. Describe how measurements
were taken and how you collected your data (units of measurement, time intervals, etc.).
Keep it simple, but make sure you include all info that someone would need if he/she wanted
to repeat your experiment. Use bullets/numbers if you like.)
Results
(This can just be a data table like this:
group 1
group 2
group 3
time point 1
observation
observation
observation
time point 2
observation
observation
observation
You could also include a graph here, if appropriate. You can also just summarize your results
instead.)
Discussion
(Interpret your results ­ Was your hypothesis supported, or should you reject it based on your
results? What could have been some sources of error? Are there any findings from this study
that might be interesting to follow up on?)
References
(List bibliographic citations (whatever format you want ­ just make it consistent) for any
references you used. Maybe you looked up some background info for your introduction.)

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